zo 1 af5145  (Affinity Biosciences)

Journal: Microorganisms

Article Title: Carbonate Buffer Mixture Alleviates Subacute Rumen Acidosis Induced by Long-Term High-Concentrate Feeding in Dairy Goats by Regulating Rumen Microbiota

doi: 10.3390/microorganisms13040945

Figure Lengend Snippet: The effects of CBM on rumen barrier in SARA dairy goats. Rumen tissues of dairy goats in Ctrl group, SARA group, and CBM group were collected, and their barrier integrity was detected. ( A ) Representative H&E-stained images of rumen tissues from three different groups (100×/200×) (n = 3). ( B ) Histopathological score of the rumen tissues. ( C – F ) The expression levels of the TJs ZO-1, Occludin, and Claudin-3 in representative rumen tissues as detected by western blotting ( C ), and the relative intensity of the three proteins using β-actin as an internal reference ( D – F ) (n = 3). * p < 0.05, ** p < 0.01 and **** p < 0.0001 indicate significant differences. One-way ANOVA ( B , D – F ) was used, and values are presented as means ± SDs ( B , D – F ).

Article Snippet: Specific antibodies including beta-actin (β-actin) (#AF7018), Zonula occludens-1 (ZO-1) (#AF5145), Occludin (#DF7504), and Claudin-3 (#AF0129) were obtained from Affinity Biosciences (Cincinnati, OH, USA).

Techniques: Staining, Expressing, Western Blot

Journal: Microorganisms

Article Title: Carbonate Buffer Mixture Alleviates Subacute Rumen Acidosis Induced by Long-Term High-Concentrate Feeding in Dairy Goats by Regulating Rumen Microbiota

doi: 10.3390/microorganisms13040945

Figure Lengend Snippet: The effects of CBM on gut barrier in SARA dairy goats. Gut tissues of dairy goats in Ctrl group, SARA group, and CBM group were collected, and their barrier integrity was detected. ( A ) Representative H&E-stained images of gut tissues from three different groups (scale bar 50 μm) (n = 3). ( B ) Histopathological score of the gut tissues. ( C – F ) The expression levels of the TJs ZO-1, Occludin, and Claudin-3 in representative gut tissues as detected by western blotting ( C ) and the relative intensity of the three proteins using β-actin as an internal reference ( D – F ) (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 indicate significant differences. One-way ANOVA ( B , D – F ) was used, and values are displayed as means ± SDs ( B , D – F ).

Article Snippet: Specific antibodies including beta-actin (β-actin) (#AF7018), Zonula occludens-1 (ZO-1) (#AF5145), Occludin (#DF7504), and Claudin-3 (#AF0129) were obtained from Affinity Biosciences (Cincinnati, OH, USA).

Techniques: Staining, Expressing, Western Blot